Publications
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Preprints
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2024
Discovery, characterization, and structure of a cofactor-independent histidine racemase from the oral pathogen Fusobacterium nucleatum. Tess Lamer, Pu Chen, Marie J. Venter, Marco J. van Belkum, Anjalee Wijewardane, Chenggang Wu, M. Joanne Lemieux, John C. Vederas. J Biol Chem In press
A structural comparison of oral SARS-CoV-2 drug candidate ibuzatrelvir complexed with main proteases (Mpro) of SARS CoV 2 and MERS-CoV Chen, Pu; Van Oers, Tayla; Arutyunova, Elena; Fischer, Conrad; Wang, Chaoxiang; Lamer, Tess; van Belkum, Marco; Young, Howard; Vederas, John; Lemieux, M Joanne. JACS Au 2024, 4, 8, 3217–3227
Elusive Protein-Glycosphingolipid Interactions Revealed by Membrane Anchor-Assisted Native Mass Spectrometry. Favell JW, Bui DT, Li J, Han L, Kitova EN, Schmidt EN, Brassard R, Kitov PI, St-Pierre Y, Mahal LK, Lemieux MJ, Macauley MS, Klassen JS. J Am Chem Soc. 2024 Aug 7;146(31):21700-21709. doi: 10.1021/jacs.4c05805. Epub 2024 Jul 25. PMID: 39052014.
Probing the formation of a hetero-dimeric membrane transport complex with dual in vitro and in silico mutagenesis. Rathod N, Lemieux MJ, Chipot C, Roux B, Young HS. Chem Sci. 2024 Aug 6;15(35):14310–22. doi: 10.1039/d4sc02915a. Epub ahead of print. PMID: 39156929; PMCID: PMC11322925.
2023
Rational Engineering of an Improved Genetically Encoded pH Sensor Based on Superecliptic pHluorin. Shen Y, Wen Y, Sposini S, Vishwanath AA, Abdelfattah AS, Schreiter ER, Lemieux MJ, de Juan-Sanz J, Perrais D, Campbell RE. ACS Sens. 2023 Aug 25;8(8):3014-3022. doi: 10.1021/acssensors.3c00484. Epub 2023 Jul 23. PMID: 37481776.
Deuteration for Metabolic Stabilization of SARS-CoV-2 Inhibitors GC373 and Nirmatrelvir. Tayla J. Van Oers, Alexia Piercey, Alexandr Belovodskiy, Béla Reiz, Bethan L. Donnelly, Wayne Vuong, M. Joanne Lemieux, James A. Nieman, Karine Auclair, and John C. Vederas. Organic Letters 2023 25 (31), 5885-5889DOI: 10.1021/acs.orglett.3c02140
Michael James, J. N. Mark Glover · Cyril M. Kay · Joanne Lemieux · Randy J. Read. Acta Crystallographica Section D Structural Biology. Sept 2023. 9
The effect of deuteration and homologation of the lactam ring of nirmatrelvir on its biochemical properties and oxidative metabolism. Arutyunova, Elena; Belovodskiy, Alexandr; Chen, Pu; Joyce, Michael; Saffran, Holly; Lu, Jimmy; Turner, Zoe; Bai, Bing; Lamer, Tess; Vederas, John; Tyrrell, D. Lorne; Lemieux, M Joanne; Nieman, James. ACS Bio & Med Chem Au2023, 3 (6), 528-541
SARS-CoV-2 Mpro Protease Variants of Concern Display Altered Viral Substrate and Cell Host Targe Galectin-8 Processing but Retain Sensitivity toward Antivirals. Chen SA, Arutyunova E, Lu J, Khan MB, Rut W, Zmudzinski M, Shahbaz S, Iyyathurai J, Moussa EW, Turner Z, Bai B, Lamer T, Nieman JA, Vederas JC, Julien O, Drag M, Elahi S, Young HS, Lemieux MJ. ACS Cent Sci.2023 Mar 21;9(4):696-708. doi: 10.1021/acscentsci.3c00054. PMID: 37122453
Rational engineering of an improved genetically encoded pH sensor based on superecliptic pHluorin. Shen, Yi; Wen, Yurong; Sposini, Silvia; Amrapali Vishwanath, Anjali; Abdelfattah, Ahmed; Schreiter, Eric; Lemieux, M Joanne; de Juan-Sanz, Jaime; Perrais, David; Campbell, Robert. ACS Sensors
2022
A sensitive and specific genetically encoded potassium ion biosensor for in vivo applications across the tree of life.Wu SY, Wen Y, Serre NBC, Laursen CCH, Dietz AG, Taylor BR, Drobizhev M, Molina RS, Aggarwal A, Rancic V, Becker M, Ballanyi K, Podgorski K, Hirase H, Nedergaard M, Fendrych M, Lemieux MJ, Eberl DF, Kay AR, Campbell RE, Shen Y. PLoS Biol. 2022 Sep 6;20(9):e3001772. doi: 10.1371/journal.pbio.3001772. Epub ahead of print. PMID: 36067248.
Acyl-CoA: diacylglycerol acyltransferase: Properties, physiological roles, metabolic engineering and intentional control G Chen, JL Harwood, MJ Lemieux, SJ Stone, RJ Weselake Prog Lipid Res. 2022 Jul 9:101181. doi: 10.1016/j.plipres.2022.101181.
Primitive Phospholamban-and Sarcolipin-like Peptides Inhibit the Sarcoplasmic Reticulum Calcium Pump SERCA Bak JJ, Aguayo-Ortiz R, Rathod N, Primeau JO, Khan MB, Robia SL, Lemieux MJ, Espinoza-Fonseca LM, Young HS. Biochemistry 2022 Jun 30. doi: 10.1021/acs.biochem.2c00246.
Crystallization of Feline Coronavirus Mpro With GC376 Reveals Mechanism of Inhibition Lu J, Chen SA, Khan MB, Brassard R, Arutyunova E, Lamer T, Vuong W, Fischer C, Young HS, Vederas JC, Lemieux MJ Front Chem. 2022 Feb 24;10:852210. doi: 10.3389/fchem.2022.852210
Novel enzyme discovery from the rumen microbiome Neves ALA, Yu J, Suzuki Y, Baez-Magana M, Arutyunova E, O'Hara E, McAllister T, Ominski KH, Lemieux MJ, Guan LL. Microbiome. 2021 Nov 23;9(1):229. doi: 10.1186/s40168-021-01147-1.
2021
A genetically encoded fluorescent biosensor for extracellular L-lactate. Yusuke Nasu, Ciaran Murphy-Royal, Yurong Wen, Jordan Haidey, Anne Schohl6, Marion R. Van Horn, M. Rosana S. Molina, Abhi Aggarwal, Shuce Zhang, Yuki Kamijo, Kaspar Podgorski, Mikhail Drobizhev, Edward S. Ruthazer, Jaideep S. Bains, M. Joanne Lemieux, Grant R. Gordon, Robert E. Campbell. Nature Comm 2021 Dec 6;12(1):7058. doi: 10.1038/s41467-021-27332-2. PMID: 34873165; PMCID: PMC8648760.
Bai B., Elena Arutyunova, Muhammad Bashir Khan, Jimmy Lu, Michael A. Joyce, Holly A. Saffran, Justin A. Shields, Appan Srinivas Kandadai, Alexandr Belovodskiy, Mostofa Hena, Wayne Vuong, Tess Lamer, Howard S. Young, John C. Vederas, D. Lorne Tyrrell, M. Joanne Lemieux, James A. Nieman. Peptidomimetic nitrile warheads as SARS-CoV-2 3CL protease inhibitors. RCS Med Chem, 2021, 12 1722-1730.
Rathod N, Bak JJ, Primeau JO, Fisher ME, Espinoza-Fonseca LM, Lemieux MJ, Young HS. Nothing Regular about the Regulins: Distinct Functional Properties of SERCA Transmembrane Peptide Regulatory Subunits. Int J Mol Sci. 2021 Aug 18;22(16):8891. doi: 10.3390/ijms22168891. PMID: 34445594; PMCID: PMC8396278.
Law L.M.J., Michael Logan, Michael Joyce, Darren Hockman, Amir Landi, Kevin Crawford, Janelle Johnson, Gerald LaChance, Holly Bandi, Justin Shield, Eve Hobart, Raelynn Brassard, Elena Arutyunova, M Joanne Lemieux, Lorne Tyrrell & Michael Houghton. SARS-COV-2 recombinant receptor-binding-domain (rbd) induces neutralising antibodies against variant strains of SARS-CoV-2 and SARS-CoV-1. Vaccine. 2021 Sep 24;39(40):5769-5779. doi: 10.1016/j.vaccine.2021.08.081. Epub 2021 Aug 26. PubMed PMID: 34481699
Bai, B., Belovodskiy, A., Hena, M., Kandadai, A.S., Joyce, M.,Saffran, H., Shields, J., Khan, M.B., Arutyunova, E., Lu, J.; Bajwa, S., Hockman, Darren; Fischer, Conrad; Lamer, Tess; Voung, Wayne; Van Belkum, Marco; Gu, Zhengxian; Lin, Fusen; Du, Yanhua; Xu, Jia; Rahim, Mohammad; Young, Howard; Vederas, John; Tyrrell, D. Lorne; Lemieux, M Joanne; Nieman, James. Peptidomimetic α-acyloxymethylketone warheads with 6-membered lactam P1 glutamine mimic: SARS-CoV-2 3CL protease inhibition, coronavirus antiviral activity and in vitro biological stability. J Med Chem 2021 Jul 9. doi: 10.1021/acs.jmedchem.1c00616.
Vuong W, Fischer C, Khan MB, van Belkum MJ, Lamer T, Willoughby KD, Lu J, Arutyunova E, Joyce MA, Saffran HA, Shields JA, Young HS, Nieman JA, Tyrrell DL, Lemieux MJ, Vederas JC. Improved SARS-CoV-2 Mpro inhibitors based on feline antiviral drug GC376: Structural enhancements, increased solubility, and micellar studies. Eur J Med Chem. 2021 May 30;222:113584. doi: 10.1016/j.ejmech.2021.113584.
Xiaocen Lu, Yurong Wen, Shuce Zhang, Wei Zhang, Yilun Chen, Yi Shen, M. Joanne Lemieux, and Robert E. Campbell. Improved Photocleavable Proteins with Faster and More Efficient Dissociation. Chemical Science, DOI: 10.1039/d1sc01059j
M’Lynn E. Fisher, Elisa Bovo, Rodrigo Aguayo-Ortiz, Ellen E. Cho2 , Marsha P. Pribadi , Michael P. Dalton, Nishadh Rathod1 , M. Joanne Lemieux , L. Michel Espinoza-Fonseca, Seth L. Robia, Aleksey V. Zima, Howard S. Young. Dwarf open reading frame (DWORF) is a direct activator of the sarcoplasmic reticulum calcium pump SERCA. eLife. 2021 Jun 2;10:e65545. doi: 10.7554/eLife.65545.
Arutyunova, E., Khan, M.B., Fischer, C., Lu, J., Lamer, T., Vuong, W., van Belkum, M.J., McKay, R.T., Tyrrell, D.L., Vederas, J.C., Young, H.S. & Lemieux, M.J. N-Terminal finger stabilizes the S1 pocket for the reversible feline drug GC376 in the SARS-CoV-2 Mpro dimer. J Mol Biol. 2021 Jun 25;433(13):167003. doi: 10.1016/j.jmb.2021.167003.
Arutyunova E., Lysyk L., Morrison M., Brooks C., Joanne Lemieux M. (2021) Expression and Purification of Human Mitochondrial Intramembrane Protease PARL. In: Schmidt-Krey I., Gumbart J.C. (eds) Structure and Function of Membrane Proteins. Methods in Molecular Biology, vol 2302. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1394-8_1
Lysyk L, Brassard, R, Arutyunova E, Siebert V, Jiang Z, Takyi E, Morrison M, Young HS, Lemberg MK, O’Donoghue AJ, M. Joanne Lemieux. Insights into the catalytic properties of the mitochondrial rhomboid protease PARL. 2021 J Biol Chem. 2021 Feb 5;296:100383. doi: 10.1016/j.jbc.2021.100383.
Lemieux MJ, Overduin M. Structure and function of proteins in membranes and nanodiscs. Biochim Biophys Acta Biomembr. 2021 Jan 1;1863(1):183445. doi: 10.1016/j.bbamem.2020.183445.
2020
Vuong, W., Khan, M.B., Fischer, C., Arutyunova, E., Lamer, T., Shields, J., Saffran, H.A., McKay, R.T., van Belkum, M.J., Joyce, M.A., Young, H.S., Tyrrell, D.L., Vederas, J.C. & Lemieux. M.J. Feline coronavirus drug inhibits the main protease of SARS-CoV-2 and blocks virus replication. Nat Commun 11, 4282 (2020).
Glaves, J.P., Primeau, J.O., Gorski, P.A., Espinoza-Fonseca, L.M., Lemieux, M.J. & Young, H.S. Interaction of a Sarcolipin Pentamer and Monomer with the Sarcoplasmic Reticulum Calcium Pump, SERCA. Biophys J 118, 518-531 (2020).
Long, W., Fatehi, M., Soni, S., Panigrahi, R., Philippaert, K., Yu, Y., Kelly, R., Boonen, B., Barr, A., Golec, D., Campbell, S.A., Ondrusova, K., Hubert, M., Baldwin, T., Lemieux, M.J. & Light, P.E. Vitamin D is an endogenous partial agonist of the transient receptor potential vanilloid 1 channel. J Physiol. 2020 Oct;598(19):4321-4338. doi: 10.1113/JP279961.
Lysyk, L., Brassard, R., Touret, N. & Lemieux, M.J. PARL Protease: A Glimpse at Intramembrane Proteolysis in the Inner Mitochondrial Membrane. J Mol Biol 432, 5052-5062 (2020).
Ouyang, Z., Felix, J., Zhou, J., Pei, Y., Ma, B., Hwang, P.M., Lemieux, M.J., Gutsche, I., Zheng, F. & Wen, Y. Trimeric structure of the mouse Kupffer cell C-type lectin receptor Clec4f. FEBS Lett 594, 189-198 (2020).
Chen, R., Ganesan, A., Okoye, I., Arutyunova, E., Elahi, S., Lemieux, M.J. & Barakat, K. Targeting B7-1 in immunotherapy. Med Res Rev. 2020 Mar;40(2):654-682. doi: 10.1002/med.21632.
2019
Ouyang Z, Zheng F, Chew JY, Pei Y, Zhou J, Wen K, Han M, Lemieux MJ, Hwang PM, Wen Y. Deciphering the activation and recognition mechanisms of Staphylococcus aureus response regulator ArlR. Nucleic Acids Res. 2019 Dec 2;47(21):11418-11429. doi: 10.1093/nar/gkz891.
Ganesan, A., Ahmed, M., Okoye, I., Arutyunova, E., Babu, D., Turnbull, W.L., Kundu, J.K., Shields, J., Agopsowicz, K.C., Xu, L., Tabana, Y., Srivastava, N., Zhang, G., Moon, T.C., Belovodskiy, A., Hena, M., Kandadai, A.S., Hosseini, S.N., Hitt, M., Walker, J., Smylie, M., West, F.G., Siraki, A.G., Lemieux, M.J., Elahi, S., Nieman, J.A., Tyrrell, D.L., Houghton, M. & Barakat, K. Comprehensive in vitro characterization of PD-L1 small molecule inhibitors. Sci Rep 9, 12392 (2019).
Bondar AN, Lemieux MJ. Reactions at biomembrane interfaces. Chemical Reviews 2019 In press. http://dx.doi.org/10.1021/acs.chemrev.8b00596.
Young HS and Lemieux MJ. Regulating the regulator. EMBO Reports 2019 EMBO Rep. 2019w Mar;20(3). pii: e47792. doi: 10.15252/embr.201947792. Epub 2019 Feb 25.
Glaves JP, Primeau JO, Espinoza-Fonseca LM, Lemieux MJ, Young HS. The phospholamban pentamer alters function of the sarcoplasmic reticulum calcium pump SERCA. Biophys J. Biophys J. 2019 Feb 19;116(4):633-647. doi: 10.1016/j.bpj.2019.01.013.
Lapek JD Jr, Jiang Z, Wozniak JM, Arutyunova E, Wang SC, Lemieux MJ, Gonzalez DJ, O'Donoghue AJ.Quantitative Multiplex Substrate Profiling of Peptidases by Mass Spectrometry. Mol Cell Proteomics. 2019 Jan 31. pii: mcp.TIR118.001099. doi: 10.1074/mcp.TIR118.001099. [Epub ahead of print]
2018
Lemieux MJ, Denault JB, Overall CM. Highlight: Frontiers in Proteolysis. Biol Chem. 2018 Nov 27;399(12):1351. doi: 10.1515/hsz-2018-0401.
Li, M.X., Gelozia, S., Danmaliki, G.I., Wen, Y., Liu, P.B., Lemieux, M.J., West, F.G., Sykes, B.D. & Hwang, P.M. The calcium sensitizer drug MCI-154 binds the structural C-terminal domain of cardiac troponin C. Biochem Biophys Rep 16, 145-151 (2018).
Panigrahi, R., Matsui, T., Song, A.H., Caldo, K.M.P., Young, H.S., Weselake, R.J. & Lemieux, M.J. Intrinsic disorder in the regulatory N-terminal domain of diacylglycerol acyltransferase 1 from Brassica napus. Sci Rep 2018 Nov 12;8(1):16665. doi: 10.1038/s41598-018-34339-1.
Friedman, R., Khalid, S., Aponte-Santamaria, C., Arutyunova, E., Becker, M., Boyd, K.J., Christensen, M., Coimbra, J.T.S., Concilio, S., Daday, C., van Eerden, F.J., Fernandes, P.A., Grater, F., Hakobyan, D., Heuer, A., Karathanou, K., Keller, F., Lemieux, M.J., Marrink, S.J., May, E.R., Mazumdar, A., Naftalin, R., Pickholz, M., Piotto, S., Pohl, P., Quinn, P., Ramos, M.J., Schiott, B., Sengupta, D., Sessa, L., Vanni, S., Zeppelin, T., Zoni, V., Bondar, A.N. & Domene, C. Understanding Conformational Dynamics of Complex Lipid Mixtures Relevant to Biology. J Membr Biol 251, 609-631 (2018).
Arutyunova E, Jiang Z, Yang J, KalupaAN, Young HS, Verhelst S, O’Donoghue A, Lemieux MJ.. An internally quenched peptide as a new model substrate for rhomboid intramembrane proteases. Biological Chem. 2018 Nov 27;399(12):1389-1397
Xu Y, Caldo KMP, Pal-Nath D, Petrie J, Ozga J, Lemieux MJ, Weselake RJ, Chen G.. Properties and Biotechnological Applications of Acyl-CoA:diacylglycerol Acyltransferase and Phospholipid:diacylglycerol Acyltransferase from Terrestrial Plants and Microalgae. Lipids. 2018 Jul;53(7):663-688. doi: 10.1002/lipd.12081.
Caldo KMP, Shen, Xu Y, Hanley-Bowdoin L, Chen G, Weselake RJ, Lemieux MJ (2018) Diacylglycerol acyltransferase 1 is activated by phosphatidate and inhibited by SnRK1-catalyzed phosphorylation. Plant J. 2018 Oct;96(2):287-299. doi: 10.1111/tpj.14029.
Lian, J., Bahitham, W., Panigrahi, R., Nelson, R., Li, L., Watts, R., Thiesen, A., Lemieux, M.J. & Lehner, R. Genetic variation in human carboxylesterase CES1 confers resistance to hepatic steatosis. Biochim Biophys Acta Mol Cell Biol Lipids1863, 688-699 (2018).
Lemieux MJ. Taking a position on intramembrane proteolysis. J Biol Chem. 2018 Mar 30;293(13):4664-4665.
2017
Smeazzetto S, Armanious GP, Moncelli MR, Bak JJ, Lemieux MJ Young HS, Tadini-Buoninsegni F. Conformational memory in the association of the transmembrane protein phospholamban with the sarcoplasmic reticulum calcium pump SERCA.J Biol Chem. 2017 Oct 29. pii: jbc.M117.794453. doi: 10.1074/jbc.M117.794453. [Epub ahead of print]. PMID: 29081402
Xu X, Chen G, Greer M, Caldo KMP, Ramakrishnan G, Shah S, Wu L, Lemieux MJ Ozga J, Weselake RJ. Multiple mechanisms contribute to increased neutral lipid accumulation in yeast producing recombinant variants of plant diacylglycerol acyltransferase 1. 2017). J Biol Chem. 2017 Oct 27;292(43):17819-17831. doi: 10.1074/jbc.M117.811489.
Caldo KMP, Acedo JZ, Panigrahi R, Vederas JC, Weselake RJ, Lemieux MJ(2017)Diacylglycerol Acyltransferase 1 Is Regulated by Its N-Terminal Domain in Response to Allosteric Effectors.. Plant Physiology. 2017. vol. 175 pp 667-680. PMID:28827454
Soueidan, O. M., Scully, T. W., Kaur, J., Panigrahi, R., Belovodskiy, A., Do, V., Matier, C. D., Lemieux MJ, Wuest, F., Cheeseman, C., and West, F. G. (2017) Fluorescent Hexose Conjugates Establish Stringent Stereochemical Requirement by GLUT5 for Recognition and Transport of Monosaccharides. ACS Chem Biol Feb 16. doi: 10.1021/acschembio.6b01101. [Epub ahead of print]PMID: 28205432
Long, W., Panigrahi, R., Panwar, P., Wong, K., D, O. N., Chen, X. Z., Lemieux, M. J., and Cheeseman, C. I. (2017) Identification of Key Residues for Urate Specific Transport in Human Glucose Transporter 9 (hSLC2A9). Scientific reports 7, 41167
Arutyunova, E., Strisovsky, K., and Lemieux, M. J. (2017) Activity Assays for Rhomboid Proteases. Methods in enzymology 584, 395-437
Arutyunova, E., Panigrahi, R., Strisovsky, K., and Lemieux, M. J. 2017) Production of Recombinant Rhomboid Proteases. Methods in enzymology 584, 255-278
2016
Arutyunova, E., Smithers, C. C., Corradi, V., Espiritu, A. C., Young, H. S., Tieleman, D. P., and Lemieux, M. J.. (2016) Probing catalytic rate enhancement during intramembrane proteolysis. Biological chemistry 397, 907-919
Panigrahi, R., Arutyunova, E., Panwar, P., Gimpl, K., Keller, S., and Lemieux, M. J. (2016) Reversible Unfolding of Rhomboid Intramembrane Proteases. Biophys J 110, 1379-1390
2015
Panigrahi, R., and Lemieux, M. J. (2015) Functional Implications of Domain Organization Within Prokaryotic Rhomboid Proteases. Advances in experimental medicine and biology 883, 107-117
Girke, C., Arutyunova, E., Syed, M., Traub, M., Mohlmann, T., and Lemieux, M. J. (2015) High yield expression and purification of equilibrative nucleoside transporter 7 (ENT7) from Arabidopsis thaliana. Biochim Biophys Acta 1850, 1921-1929
Cournia, Z., Allen, T. W., Andricioaei, I., Antonny, B., Baum, D., Brannigan, G., Buchete, N. V., Deckman, J. T., Delemotte, L., Del Val, C., Friedman, R., Gkeka, P., Hege, H. C., Henin, J., Kasimova, M. A., Kolocouris, A., Klein, M. L., Khalid, S., Lemieux, M. J., Lindow, N., Roy, M., Selent, J., Tarek, M., Tofoleanu, F., Vanni, S., Urban, S., Wales, D. J., Smith, J. C., and Bondar, A. N. (2015) Membrane Protein Structure, Function, and Dynamics: a Perspective from Experiments and Theory. The Journal of membrane biology 248, 611-640
Long, W., Panwar, P., Witkowska, K., Wong, K., O'Neill, D., Chen, X. Z., Lemieux, M. J., and Cheeseman, C. I. (2015) Critical Roles of Two Hydrophobic Residues within Human Glucose Transporter 9 (hSLC2A9) in Substrate Selectivity and Urate Transport. J Biol Chem 290, 15292-15303
Caldo, K. M., Greer, M. S., Chen, G., Lemieux, M. J., and Weselake, R. J. (2015) Purification and properties of recombinant Brassica napus diacylglycerol acyltransferase 1. FEBS Lett 589, 773-778
2014
Arutyunova, E., Panwar, P., Skiba, P. M., Gale, N., Mak, M. W., and Lemieux, M. J. (2014) Allosteric regulation of rhomboid intramembrane proteolysis. EMBO J 33, 1869-1881
Brooks, C. L., Arutyunova, E., and Lemieux, M. J. (2014) The structure of lactoferrin-binding protein B from Neisseria meningitidis suggests roles in iron acquisition and neutralization of host defences. Acta crystallographica. Section F, Structural biology communications 70, 1312-1317
Niopek-Witz, S., Deppe, J., Lemieux, M. J., and Mohlmann, T. (2014) Biochemical characterization and structure-function relationship of two plant NCS2 proteins, the nucleobase transporters NAT3 and NAT12 from Arabidopsis thaliana. Biochim Biophys Acta 1838, 3025-3035
Panwar, P., and Lemieux, M. J. (2014) Expression and Purification of Haemophilus influenzae Rhomboid Intramembrane Protease GlpG for Structural Studies. Current protocols in protein science / editorial board, John E. Coligan ... [et al.] 76, 29 29 21-25
Witz, S., Panwar, P., Schober, M., Deppe, J., Pasha, F. A., Lemieux, M. J., and Mohlmann, T. (2014) Structure-function relationship of a plant NCS1 member--homology modeling and mutagenesis identified residues critical for substrate specificity of PLUTO, a nucleobase transporter from Arabidopsis. PloS One 9, e91343
2013
Brooks, CL, Lemieux MJ. Untangling the structure function relationship of rhomboid protease. BBA Biomembranes. Invited review, special edition. 2013 Dec;1828(12):2862-72. doi: 10.1016/j.bbamem.2013.05.003. Review.
Brooks, C. L., Morrison, M., and Joanne Lemieux, M. (2013) Rapid expression screening of eukaryotic membrane proteins in Pichia pastoris. Protein science : a publication of the Protein Society 22, 425-433
Lazareno-Saez, C., Arutyunova, E., Coquelle, N., M. Joanne Lemieux. Domain swapping in the cytoplasmic domain of E. coli rhomboid protease. J Mol Biol. 2013. Apr 12;425(7):1127-42. doi: 10.1016/j.jmb.2013.01.019.
2012
Padmapriya Sampathkumar, Michelle W. Mak, Sarah J. Fischer-Witholt, Emmanuel Guigard, Cyril M. Kay, M.Joanne Lemieux. Oligomeric state study of prokaryotic rhomboid proteases. Biochim Biophys Acta - Biomembranes, Dec 2012; 1818(12):3090-7. (2012)
Arutyunova, E., Brooks, C.L., Beddek, A., Mak, M.W., Schryvers A.B. Lemieux. MJ. Crystal structure of the N-lobe of lactoferrin binding protein B from Moraxella bovis. Biochem Cell Biol. Jun;90(3):351-61. (2012).
2011
Brooks, C.L., Lazareno-Saez, C., Lamoureux, J.S, Mak, M.W. and Lemieux, M.J. Structural evidence for substrate gating indicates a new mechanism for a rhomboid intramembrane peptidase. J Mol Biol. Apr 15;407(5):687-97. (2011)
Lazareno-Saez, C, Brooks, CL., Lemieux, M.J. Structural Comparison of Substrate Entry Gate for Rhomboid Intramembrane Peptidases. Biochem and Cell Bio. 89, 216-223. (2011)
2008
Lemieux, M.J. (2008). A perspective on the structural studies of inner membrane electrochemical potential-driven transporters. Biochim Biophys Acta 1778, 1805-1813.
POST-DOCTORAL PUBLICATIONS (University of Alberta, Edmonton, AB)
Lemieux, M. J., 2007, Eukaryotic major facilitator superfamily transporter modeling based on the prokaryotic GlpT crystal structure: Mol Membr Biol, v. 24, p.333-41.
Lemieux, M. J., S. J. Fischer, M. M. Cherney, K. S. Bateman, and M. N. James, 2007, The crystal structure of the rhomboid peptidase from Haemophilus influenzae provides insight into intramembrane proteolysis: Proc Natl Acad Sci U S A, v. 104, p. 750-4.
Lemieux, M. J., B. L. Mark, M. M. Cherney, S. G. Withers, D. J. Mahuran, and M. N. James, 2006, Crystallographic structure of human beta-hexosaminidase A: interpretation of Tay-Sachs mutations and loss of GM2 ganglioside hydrolysis: J Mol Biol, v. 359, p. 913-29.
Lemieux, M. J., C. Ference, M. M. Cherney, M. Wang, C. Garen, and M. N. James, 2005, The crystal structure of Rv0793, a hypothetical monooxygenase from M. tuberculosis: J Struct Funct Genomics, v. 6, p. 245-57.
Slepkov, E.R., Chow, S., Lemieux, M.J., and Fliegel, L. (2004). Proline residues in transmembrane segment IV are critical for activity, expression and targeting of the Na+/H+ exchanger isoform 1. Biochem J 379, 31-38.
DOCTORAL PUBLICATIONS (New York University, NY, NY, USA)
Lemieux, M. J., Y. Huang, and N. Wang da, 2005, Crystal structure and mechanism of GlpT, the glycerol-3-phosphate transporter from E. coli: J Electron Microsc (Tokyo), v. 54 Suppl 1, p. i43-i46.
Lemieux, M. J., Y. Huang, and D. N. Wang, 2004, Glycerol-3-phosphate transporter of Escherichia coli: structure, function and regulation: Res Microbiol, v. 155, p. 623-9.
Lemieux, M. J., Y. Huang, and D. N. Wang, 2004, The structural basis of substrate translocation by the Escherichia coli glycerol-3-phosphate transporter: a member of the major facilitator superfamily: Curr Opin Struct Biol, v. 14, p. 405-12.
Huang, Y., M. J. Lemieux, J. Song, M. Auer, and D. N. Wang, 2003, Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli: Science, v. 301, p. 616-20.
Lemieux, M. J., J. Song, M. J. Kim, Y. Huang, A. Villa, M. Auer, X. D. Li, and D. N. Wang, 2003, Three-dimensional crystallization of the Escherichia coli glycerol-3-phosphate transporter: a member of the major facilitator superfamily: Protein Sci, v. 12, p. 2748-56.
Wang, D. N., M. J. Lemieux, and J. M. Boulter, 2003a, Purification and characterization of transporter proteins from human erythrocyte membrane: Methods Mol Biol, v. 228, p. 239-55.
Wang, D. N., M. Safferling, M. J. Lemieux, H. Griffith, Y. Chen, and X. D. Li, 2003, Practical aspects of overexpressing bacterial secondary membrane transporters for structural studies: Biochim Biophys Acta, v. 1610, p. 23-36.
Lemieux, M. J., R. A. Reithmeier, and D. N. Wang, 2002, Importance of detergent and phospholipid in the crystallization of the human erythrocyte anion-exchanger membrane domain: J Struct Biol, v. 137, p. 322-32.
Auer, M., M. J. Kim, M. J. Lemieux, A. Villa, J. Song, X. D. Li, and D. N. Wang, 2001, High-yield expression and functional analysis of Escherichia coli glycerol-3-phosphate transporter: Biochemistry, v. 40, p. 6628-35.
MASTER'S AND B.Sc. PUBLICATIONS (Dalhousie University, Halifax, NS)
Lemieux, M. J., C. Mezei, and W. C. Breckenridge, 1996, Secretion and processing of apolipoprotein A-I in the avian sciatic nerve during development: J Neurosci Res, v. 44, p. 594-605.
Lemieux, M. J., C. Mezei, and W. C. Breckenridge, 1995, Biosynthesis and compartmentalization of Po, apolipoprotein A-I, and lipids in the myelinating chick sciatic nerve: Neurochem Res, v. 20, p. 1239-48.
Lemieux, J., Giannoulis, S., Breckenridge, W.C., and Mezei, C. (1995). Post-translational modifications of apolipoprotein A-I and Po proteins in the avian peripheral nerve. Neurochem Res 20, 269-278.
LeBlanc, A.C., Pringle, J., Lemieux, J., Poduslo, J.F., and Mezei, C. (1992). Regulation of 2',3'-cyclic nucleotide phosphodiesterase gene expression in experimental peripheral neuropathies. Brain Res Mol Brain Res 15, 40-46.